By P.F. Stanbury, A. Whitaker

ISBN-10: 0750645016

ISBN-13: 9780750645010

This moment version has been completely up-to-date to incorporate contemporary advances and advancements within the box of fermentation know-how, concentrating on business purposes. The e-book now covers new features reminiscent of recombinant DNA suggestions within the development of commercial micro-organisms, and contains complete details on fermentation media, sterilization techniques, inocula, and fermenter layout. Chapters on effluent remedy and fermentation economics also are included. The textual content is supported by way of quite a few transparent, informative diagrams.The publication is of serious curiosity to ultimate yr and post-graduate scholars of utilized biology, biotechnology, microbiology, biochemical and chemical engineering.

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27) III where R cont is the output of the culture (g biomass dm -3 h -1), t iii is the time period prior to the establishment of a steady state and includes vessel preparation, sterilization and operation in batch culture prior to continuous operation, and T is the time period during which steadystate conditions prevail. The term Di increases with increasing dilution rate until it reaches a maximum value, after which any further increase in D results in a decrease in Di, as illustrated in Fig. 9.

With increasing dilution rate, the residual substrate concentration increases only slightly until D approaches JLmax when s increases significantly. , and the variable, SR; the larger S R the closer is DedI to JL max . However, JL max cannot be achieved in a simple steady state chemostat because substrate limited conditions must always prevail. 5 illustrates the continuous culture behaviour of a hypothetical bacterium with a high K, for the limiting substrate compared with the initial limiting substrate concentration.

35) an increase in JL. Subsequently, as feeding continues, the growth rate will decline gradually as biomass increases and approaches the maximum sustainable in the vessel once more, at which point the culture may be diluted again. Dilution would be achieved by withdrawing culture and refilling to the original level with sterile water or medium not containing the feed substrate. Application of fed-batch culture The use of fed-batch culture by the fermentation industry takes advantage of the fact that the concentration of the limiting substrate may be maintained at a very low level, thus avoiding the repressive effects of 29 Principles of Fermentation Technology, 2nd Edn.

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Principles of Fermentation Technology by P.F. Stanbury, A. Whitaker


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