By Taosheng Chen

ISBN-10: 1420070509

ISBN-13: 9781420070507

The advance of compatible assays, the combination of acceptable expertise, and the powerful administration of the fundamental infrastructure are all serious to the luck of any high-throughput screening (HTS) pastime. notwithstanding, few scientists have the multidisciplinary event had to keep an eye on all features of an HTS drug discovery venture. a realistic consultant to Assay improvement and High-Throughput Screening in Drug Discovery integrates the adventure of various specialists who provide primary and useful counsel throughout a number of events. The booklet first discusses assay advancements for very important objective periods comparable to protein kinases and phosphatases, proteases, nuclear receptors, G protein-coupled receptors, ion channels, and warmth surprise proteins. It subsequent examines assay advancements for phone viability, apoptosis, and infectious illnesses. The participants discover the applying of rising applied sciences and platforms, together with image-based excessive content material screening, RNA interference, and first cells. ultimately, they speak about the fundamental elements of the built-in HTS approach, similar to screening automation, compound library administration, the screening of average items from botanical assets, and screening informatics. Designed to inspire researchers to convey extra advances to the sector, this quantity offers useful suggestions on tips on how to begin, validate, optimize, and deal with a bioassay meant to display huge collections of compounds. Drawing at the wisdom from specialists actively fascinated about assay improvement and HTS, this can be a source that's either finished and concentrated.

Show description

Read Online or Download A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery (Critical Reviews in Combinatorial Chemistry) PDF

Best pharmacy books

Handbook of Preformulation: Chemical, Biological, and by Sarfaraz K. Niazi PDF

Preformulation reviews are the actual, chemical, and organic stories had to represent a drug substance for permitting the correct layout of a drug product, while the effectiveness of a drug product is set throughout the formula reports section. although the 2 disciplines overlap in perform, each one is a considerably specific part of latest drug improvement.

Download e-book for iPad: Ethnopharmacology of Medicinal Plants: Asia and the Pacific by Christophe Wiart

For the 1st time within the historical past of medicinal plant study, Ethnopharmacology of Medicinal crops: Asia and the Pacific sheds mild at the pharmacological potentials of 1 of the main exiting and significantly wealthy resources of power medicines: the medicinal vegetation of the Pacific Rim. Encompassing greater than 6000 species of plant which are nearly unexplored for pharmacology, this quantity offers leading edge details at the pharmacological houses and healing potentials of medicinal herbs utilized in the Asia-Pacific quarter opposed to irritation, CNS illnesses, and melanoma.

Edgar Jacoby's Computational Chemogenomics PDF

This ebook makes a speciality of functions of compound library layout and digital screening to extend the bioactive chemical house, to focus on hopping of chemotypes to spot synergies inside of similar drug discovery initiatives or to repurpose recognized medicinal drugs, to suggest mechanism of motion of compounds, or to spot off-target results by means of cross-reactivity research.

Additional resources for A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery (Critical Reviews in Combinatorial Chemistry)

Sample text

2003). , SAGIAN from Beckman Instruments). The disadvantage of using purely statistical parameters in assay optimization is that these systems do not take into account the desired physiological or biochemical mechanism of action in determining optimal reagent concentration. For example, the optimal substrate concentration to use in an enzyme assay may not necessarily be the one that gives the best statistics with respect to reproducibility. The optimal salt concentration required for reproducibility may be far different from physiological concentration.

Nat. Rev. Drug Discov. 3, 1001–1010. Y. K. Shoichet. 2006. A detergent-based assay for the detection of promiscuous inhibitors. Nat. Protoc. 1, 550–553. F. and S. Ferrand. 2008. Scintillation proximity assays in high-throughput screening. Assay Drug Dev. Technol. 6, 433–455. F. et al. 2002. A comparison of AlphaScreen, TR-FRET, and TRF as assay methods for FXR nuclear receptors. J. Biomol. Screen. 7, 3–10. F. and A. Schmid. 2007. Farnesyl pyrophosphate synthase: real-time kinetics and inhibition by nitrogen-containing bisphosphonates in a scintillation assay.

Assay Drug Dev. Technol. 2, 153–160. R. 2006. Principles of Fluorescence Spectroscopy. 3rd ed. Berlin: Springer. S. and Q. Wang. 2007. Seeing is believing: peptide-based fluorescent sensors of protein tyrosine kinase activity. Chem. BioChem. 8, 373–378. F. Y. Zhang. 2001. Assays for protein-tyrosine phosphatases. Meth. Enzymol. 345, 507–518. K. 2003. Assays to measure the activation of membrane tyrosine kinase receptors: focus on cellular methods. Curr. Opin. Drug Discov. Devel. 6, 760–765. I. P.

Download PDF sample

A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery (Critical Reviews in Combinatorial Chemistry) by Taosheng Chen

by Joseph

Rated 4.58 of 5 – based on 47 votes